October 1, 2014 by Becky Brown


I was involved in a recently published paper reporting on the ‘Development and validation of an OECD reproductive toxicity test guideline with the pond snail Lymnaea stagnalis (Mollusca; Gastropoda)’. This describes a draft protocol for a 56 day reproduction test and presents inter-comparison results from seven laboratories with cadmium and tributyltin as reference chemicals. The studies were conducted using adult snails (L. stagnalis is a simultaneous hermaphrodite) in a semi-static test design in which the numbers of clutches per snail and numbers of eggs per clutch were recorded. Individual fecundity over the 56 days was the primary endpoint for the test. This pre-validation was considered to be a success by the OECD and the test is now undergoing further validation with 14 laboratories from Europe and North and South America.

For more information on this publication or on invertebrate testing in general please contact me.